Peptide reconstitution calculator
Reconstitution is dissolving a lyophilized (freeze-dried) peptide in bacteriostatic water so it can be measured accurately. Enter your numbers to get the concentration, the exact volume to draw, and the equivalent units on a U-100 insulin syringe. For laboratory and research use.
How the math works
Concentration is the peptide amount divided by the water volume. 10 mg in 2 ml is 5 mg/ml, or 5000 mcg/ml. Draw volume is your target amount divided by the concentration: 250 mcg divided by 5000 mcg/ml is 0.05 ml. Since a U-100 syringe holds 100 units per ml, 0.05 ml is 5 units.
Tips for accurate reconstitution
Add the bacteriostatic water slowly down the side of the vial rather than directly onto the powder. Swirl gently, do not shake. Store the reconstituted vial at 2 to 8 degrees Celsius. More water makes each unit easier to measure but dilutes the concentration, so pick a water volume that puts your target amount in an easy-to-read range on the syringe.
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Browse the catalogFAQ
What is bacteriostatic water?
Sterile water with 0.9 percent benzyl alcohol added to inhibit bacterial growth, used to reconstitute lyophilized peptides for research.
How many units is 250 mcg?
It depends on the concentration. With a 10 mg vial in 2 ml, 250 mcg is 0.05 ml, or 5 units on a U-100 syringe. Use the calculator to compute your own numbers.
Does more water change the dose?
No. More water lowers the concentration, so you draw a larger volume for the same amount. The amount of peptide is unchanged, only how much liquid it is dissolved in.
Related: How to reconstitute a peptide · How to read a COA